Hand Hygiene in the Practice of Nursing Essay Example | Topics and Well Written Essays - 3000 words

Hand Hygiene in the Practice of Nursing - Essay Example From this discussion it is clear that the nursing code of conduct stipulates that effective communication entails: Respect to patients’ confidentiality. Some cultures do not allow sharing of certain information the practitioner should know the patients’ culture, religion and essential background in advance. Nurses should practice keen listening skills, use of non-verbal communication and to apply a professional language when dealing with patients. In addition, they should also have good writing skill in the case of record keeping. According to McCabe and Timmins, effective communication in the practice of nursing results in Improvement in, patients' conditions; increase in rates of recovery and patients’ satisfaction with nursing services.This essay highlights that  an advanced beginner demonstrates levels of acceptable performance. This category of learners has gained prior experience in relation to actual circumstances and has recognized the relevance of thes e components. At this stage, actions are guided by gained principles from prior experience. The first few weeks of my study mainly focused on infection prevention through hand hygiene. During this period, the reporter learnt that, mere negligence of failing to disinfect hands before attending to a patient can lead to negative consequences. Hands are the main causes of health care associated infections. Healthcare associated infections (HCAI) can result in advanced illness, slow recovery rate, increase in patients’ level of stress.... Some cultures do not allow sharing of certain information the practitioner should know the patients’ culture, religion and essential background in advance. Nurses should practice keen listening skills, use of non-verbal communication and to apply a professional language when dealing with patients (Nursing Council of New Zealand 2012). In addition, they should also have good writing skill in the case of record keeping. According to McCabe and Timmins (2006), effective communication in the practice of nursing results in Improvement in, patients' conditions; increase in rates of recovery and patients’ satisfaction with nursing services. In this study I will discuss my learning from a novice to an advanced beginner in the practice of hand hygiene in nursing. I will outline how Gibb’s cycle of reflection helped in my learning process. The importance of hand hygiene; the latest policies in hand washing and how Blooms taxonomy of learning helped in gaining; new knowledg e, new skills, and new attitude. DESCRIPTION: FROM NOVICE TO ADVANCED BEGINNER At the time, I commenced my undergraduate degree in nursing on 27th march 2013; my knowledge in this field was quit vague. I thought of nursing as a routine practice to patients’ recovery as I had previously witnessed in hospitals. Health practitioners I encounter in health facilities do not take the issue of hand hygiene as serious as it should be. It had never crossed my mind that hand hygiene was such an integral part of nursing. In the initial days of my learning, I did not consider decontaminating my hands before I attend to a patient. According to Benner (2001), there are five levels in a nurse’s career experience, this include; novice, advance beginner, competent, proficient, and expert. Novice refers to a beginner

Protein Extraction and Gel Electrophoresis Lab Report

Protein Extraction and Gel Electrophoresis - Lab Report Example The sodium dodecyl sulfate coats the proteins in proportion to their molecular weight and then confers the same negative electrical charge across all proteins in the sample. The rate of migration of a polypeptide in SDS-PAGE is inversely proportional to the logarithm of its molecular weight. This means that the larger the polypeptide, the slower it migrates in a gel. The molecular weight is determined by comparing the migration of protein spots to the migration of standards. Plots of log molecular weight versus the migration distance are reasonably linear. The proteins separated by SDS-PAGE are often recovered in a procedure that involves localizing the protein of interest on the gel following SDS-PAGE, eluting the protein from the gel, removing the sodium dodecyl sulfate from the eluted sample, and finally renaturing the protein for subsequent analysis. Proteins that are eluted from gels are used in varied downstream applications successfully, such as protein chemistry, determinatio n of amino acid composition, identification of polypeptides that correspond to specific enzyme activity, and other purposes.The analysis of protein concentrations is a significant assay in biochemistry research. The Bradford assay is one of the most widely used methods to determine concentrations of protein, relative to a standard. The technique is based on the formation of a complex between proteins in solution and the dye. This assay is commended for overall use, particularly for assessing concentrations of proteins for gel electrophoresis.... The proteins separated by SDS-PAGE are often recovered in a procedure that involves localizing the protein of interest on the gel following SDS-PAGE, eluting the protein from the gel, removing the sodium dodecyl sulfate from the eluted sample, and finally renaturing the protein for subsequent analysis. Proteins that are eluted from gels are used in varied downstream applications successfully, such as protein chemistry, determination of amino acid composition, identification of polypeptides that correspond to specific enzyme activity, and other purposes. The analysis of protein concentrations is a significant assay in biochemistry research. The Bradford assay is one of the most widely used method to determine concentrations of protein, relative to a standard. The technique is based on the formation of a complex between proteins in solution and the dye, Brilliant Blue G. This assay is commended for overall use, particularly for assessing concentrations of proteins for gel electrophores is. It is based on observations that absorbance maximum for acidic mixtures of Coomassie Brilliant Blue G-250 that do shift from 465 nm up to 595 nm at a time when protein binding occurs. The assay is effective because of the extermination coefficient of the albumin-dye complex solution is usually constant over a range of 10-fold concentration (Westermeier, Naven & Ho?pker, 2008). The dye reacts mainly with arginine residues but less with histidine, lysine, tryptophan, tyrosine, and phenylalanine residues. Seemingly, this examination is not all that perfect for acidic or basic proteins. However, it is somewhat sensitive to the bovine serum albumin, even more than most proteins, by a factor of two. Gamma globulin (IgG) is the protein standard of preference. The objective of this